Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs
Identifieur interne : 000576 ( Main/Exploration ); précédent : 000575; suivant : 000577Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs
Auteurs : Callie Ridenour [États-Unis] ; Adam Johnson [États-Unis] ; Emily Winne [États-Unis] ; Jaber Hossain [États-Unis] ; Guaniri Mateu-Petit [États-Unis] ; Amanda Balish [États-Unis] ; Wanda Santana [États-Unis] ; Taejoong Kim [États-Unis] ; Charles Davis [États-Unis] ; Nancy J. Cox [États-Unis] ; John R. Barr [États-Unis] ; Ruben O. Donis [États-Unis] ; Julie Villanueva [États-Unis] ; Tracie L. Williams [États-Unis] ; Li-Mei Chen [États-Unis]Source :
- Influenza and Other Respiratory Viruses [ 1750-2640 ] ; 2015.
Abstract
The emergence of avian influenza A(H7N9) virus in poultry causing zoonotic human infections was reported on March 31, 2013. Development of A(H7N9) candidate vaccine viruses (CVV) for pandemic preparedness purposes was initiated without delay. Candidate vaccine viruses were derived by reverse genetics using the internal genes of A/Puerto/Rico/8/34 (PR8). The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.
Two CVVs derived by reverse genetics were serially passaged in embryonated eggs to improve the hemagglutinin (HA) antigen yield. The total viral protein and HA antigen yields of six egg-passaged CVVs were determined by the BCA assay and isotope dilution mass spectrometry (IDMS) analysis, respectively. CVVs were antigenically characterized by hemagglutination inhibition (HI) assays with ferret antisera.
Improvement of total viral protein yield was observed for the six egg-passaged CVVs; HA quantification by IDMS indicated approximately a twofold increase in yield of several egg-passaged viruses as compared to that of the parental CVV. Several different amino acid substitutions were identified in the HA of all viruses after serial passage. However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.
If influenza A(H7N9) viruses were to acquire sustained human-to-human transmissibility, the improved HA yield of the egg-passaged CVVs generated in this study could expedite vaccine manufacturing for pandemic mitigation.
Url:
DOI: 10.1111/irv.12322
PubMed: 25962412
PubMed Central: 4548996
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000034
- to stream Pmc, to step Curation: 000034
- to stream Pmc, to step Checkpoint: 000410
- to stream Ncbi, to step Merge: 000B63
- to stream Ncbi, to step Curation: 000B63
- to stream Ncbi, to step Checkpoint: 000B63
- to stream Main, to step Merge: 000577
- to stream Main, to step Curation: 000576
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs</title>
<author><name sortKey="Ridenour, Callie" sort="Ridenour, Callie" uniqKey="Ridenour C" first="Callie" last="Ridenour">Callie Ridenour</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Johnson, Adam" sort="Johnson, Adam" uniqKey="Johnson A" first="Adam" last="Johnson">Adam Johnson</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Winne, Emily" sort="Winne, Emily" uniqKey="Winne E" first="Emily" last="Winne">Emily Winne</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="au3"><institution>Battelle Memorial Institute</institution>
<addr-line>Atlanta, Georgia, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hossain, Jaber" sort="Hossain, Jaber" uniqKey="Hossain J" first="Jaber" last="Hossain">Jaber Hossain</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mateu Petit, Guaniri" sort="Mateu Petit, Guaniri" uniqKey="Mateu Petit G" first="Guaniri" last="Mateu-Petit">Guaniri Mateu-Petit</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Balish, Amanda" sort="Balish, Amanda" uniqKey="Balish A" first="Amanda" last="Balish">Amanda Balish</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Santana, Wanda" sort="Santana, Wanda" uniqKey="Santana W" first="Wanda" last="Santana">Wanda Santana</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Taejoong" sort="Kim, Taejoong" uniqKey="Kim T" first="Taejoong" last="Kim">Taejoong Kim</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Davis, Charles" sort="Davis, Charles" uniqKey="Davis C" first="Charles" last="Davis">Charles Davis</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cox, Nancy J" sort="Cox, Nancy J" uniqKey="Cox N" first="Nancy J" last="Cox">Nancy J. Cox</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Barr, John R" sort="Barr, John R" uniqKey="Barr J" first="John R" last="Barr">John R. Barr</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O" last="Donis">Ruben O. Donis</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Villanueva, Julie" sort="Villanueva, Julie" uniqKey="Villanueva J" first="Julie" last="Villanueva">Julie Villanueva</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Williams, Tracie L" sort="Williams, Tracie L" uniqKey="Williams T" first="Tracie L" last="Williams">Tracie L. Williams</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25962412</idno>
<idno type="pmc">4548996</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548996</idno>
<idno type="RBID">PMC:4548996</idno>
<idno type="doi">10.1111/irv.12322</idno>
<date when="2015">2015</date>
<idno type="wicri:Area/Pmc/Corpus">000034</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000034</idno>
<idno type="wicri:Area/Pmc/Curation">000034</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000034</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000410</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000410</idno>
<idno type="wicri:Area/Ncbi/Merge">000B63</idno>
<idno type="wicri:Area/Ncbi/Curation">000B63</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000B63</idno>
<idno type="wicri:doubleKey">1750-2640:2015:Ridenour C:development:of:influenza</idno>
<idno type="wicri:Area/Main/Merge">000577</idno>
<idno type="wicri:Area/Main/Curation">000576</idno>
<idno type="wicri:Area/Main/Exploration">000576</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs</title>
<author><name sortKey="Ridenour, Callie" sort="Ridenour, Callie" uniqKey="Ridenour C" first="Callie" last="Ridenour">Callie Ridenour</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Johnson, Adam" sort="Johnson, Adam" uniqKey="Johnson A" first="Adam" last="Johnson">Adam Johnson</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Winne, Emily" sort="Winne, Emily" uniqKey="Winne E" first="Emily" last="Winne">Emily Winne</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="au3"><institution>Battelle Memorial Institute</institution>
<addr-line>Atlanta, Georgia, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hossain, Jaber" sort="Hossain, Jaber" uniqKey="Hossain J" first="Jaber" last="Hossain">Jaber Hossain</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mateu Petit, Guaniri" sort="Mateu Petit, Guaniri" uniqKey="Mateu Petit G" first="Guaniri" last="Mateu-Petit">Guaniri Mateu-Petit</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Balish, Amanda" sort="Balish, Amanda" uniqKey="Balish A" first="Amanda" last="Balish">Amanda Balish</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Santana, Wanda" sort="Santana, Wanda" uniqKey="Santana W" first="Wanda" last="Santana">Wanda Santana</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Taejoong" sort="Kim, Taejoong" uniqKey="Kim T" first="Taejoong" last="Kim">Taejoong Kim</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Davis, Charles" sort="Davis, Charles" uniqKey="Davis C" first="Charles" last="Davis">Charles Davis</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cox, Nancy J" sort="Cox, Nancy J" uniqKey="Cox N" first="Nancy J" last="Cox">Nancy J. Cox</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Barr, John R" sort="Barr, John R" uniqKey="Barr J" first="John R" last="Barr">John R. Barr</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O" last="Donis">Ruben O. Donis</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Villanueva, Julie" sort="Villanueva, Julie" uniqKey="Villanueva J" first="Julie" last="Villanueva">Julie Villanueva</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Williams, Tracie L" sort="Williams, Tracie L" uniqKey="Williams T" first="Tracie L" last="Williams">Tracie L. Williams</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution>
<addr-line>Atlanta, GA, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Atlanta, GA</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Influenza and Other Respiratory Viruses</title>
<idno type="ISSN">1750-2640</idno>
<idno type="eISSN">1750-2659</idno>
<imprint><date when="2015">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The emergence of avian influenza A(H7N9) virus in poultry causing zoonotic human infections was reported on March 31, 2013. Development of A(H7N9) candidate vaccine viruses (CVV) for pandemic preparedness purposes was initiated without delay. Candidate vaccine viruses were derived by reverse genetics using the internal genes of A/Puerto/Rico/8/34 (PR8). The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.</p>
</sec>
<sec><title>Methods</title>
<p>Two CVVs derived by reverse genetics were serially passaged in embryonated eggs to improve the hemagglutinin (HA) antigen yield. The total viral protein and HA antigen yields of six egg-passaged CVVs were determined by the BCA assay and isotope dilution mass spectrometry (IDMS) analysis, respectively. CVVs were antigenically characterized by hemagglutination inhibition (HI) assays with ferret antisera.</p>
</sec>
<sec><title>Results</title>
<p>Improvement of total viral protein yield was observed for the six egg-passaged CVVs; HA quantification by IDMS indicated approximately a twofold increase in yield of several egg-passaged viruses as compared to that of the parental CVV. Several different amino acid substitutions were identified in the HA of all viruses after serial passage. However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.</p>
</sec>
<sec><title>Conclusions</title>
<p>If influenza A(H7N9) viruses were to acquire sustained human-to-human transmissibility, the improved HA yield of the egg-passaged CVVs generated in this study could expedite vaccine manufacturing for pandemic mitigation.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Liu, D" uniqKey="Liu D">D Liu</name>
</author>
<author><name sortKey="Shi, W" uniqKey="Shi W">W Shi</name>
</author>
<author><name sortKey="Shi, Y" uniqKey="Shi Y">Y Shi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kilbourne, Ed" uniqKey="Kilbourne E">ED Kilbourne</name>
</author>
<author><name sortKey="Schulman, Jl" uniqKey="Schulman J">JL Schulman</name>
</author>
<author><name sortKey="Schild, Gc" uniqKey="Schild G">GC Schild</name>
</author>
<author><name sortKey="Schloer, G" uniqKey="Schloer G">G Schloer</name>
</author>
<author><name sortKey="Swanson, J" uniqKey="Swanson J">J Swanson</name>
</author>
<author><name sortKey="Bucher, D" uniqKey="Bucher D">D Bucher</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Harvey, R" uniqKey="Harvey R">R Harvey</name>
</author>
<author><name sortKey="Wheeler, Jx" uniqKey="Wheeler J">JX Wheeler</name>
</author>
<author><name sortKey="Wallis, Cl" uniqKey="Wallis C">CL Wallis</name>
</author>
<author><name sortKey="Robertson, Js" uniqKey="Robertson J">JS Robertson</name>
</author>
<author><name sortKey="Engelhardt, Og" uniqKey="Engelhardt O">OG Engelhardt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Abt, M" uniqKey="Abt M">M Abt</name>
</author>
<author><name sortKey="De Jonge, J" uniqKey="De Jonge J">J de Jonge</name>
</author>
<author><name sortKey="Laue, M" uniqKey="Laue M">M Laue</name>
</author>
<author><name sortKey="Wolff, T" uniqKey="Wolff T">T Wolff</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jing, X" uniqKey="Jing X">X Jing</name>
</author>
<author><name sortKey="Phy, K" uniqKey="Phy K">K Phy</name>
</author>
<author><name sortKey="Li, X" uniqKey="Li X">X Li</name>
</author>
<author><name sortKey="Ye, Z" uniqKey="Ye Z">Z Ye</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Robertson, Js" uniqKey="Robertson J">JS Robertson</name>
</author>
<author><name sortKey="Nicolson, C" uniqKey="Nicolson C">C Nicolson</name>
</author>
<author><name sortKey="Harvey, R" uniqKey="Harvey R">R Harvey</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E Hoffmann</name>
</author>
<author><name sortKey="Krauss, S" uniqKey="Krauss S">S Krauss</name>
</author>
<author><name sortKey="Perez, D" uniqKey="Perez D">D Perez</name>
</author>
<author><name sortKey="Webby, R" uniqKey="Webby R">R Webby</name>
</author>
<author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dong, J" uniqKey="Dong J">J Dong</name>
</author>
<author><name sortKey="Matsuoka, Y" uniqKey="Matsuoka Y">Y Matsuoka</name>
</author>
<author><name sortKey="Maines, Tr" uniqKey="Maines T">TR Maines</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Williams, Tl" uniqKey="Williams T">TL Williams</name>
</author>
<author><name sortKey="Luna, L" uniqKey="Luna L">L Luna</name>
</author>
<author><name sortKey="Guo, Z" uniqKey="Guo Z">Z Guo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Santana, Wi" uniqKey="Santana W">WI Santana</name>
</author>
<author><name sortKey="Williams, Tl" uniqKey="Williams T">TL Williams</name>
</author>
<author><name sortKey="Winne, Ek" uniqKey="Winne E">EK Winne</name>
</author>
<author><name sortKey="Pirkle, Jl" uniqKey="Pirkle J">JL Pirkle</name>
</author>
<author><name sortKey="Barr, Jr" uniqKey="Barr J">JR Barr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Klimov, A" uniqKey="Klimov A">A Klimov</name>
</author>
<author><name sortKey="Balish, A" uniqKey="Balish A">A Balish</name>
</author>
<author><name sortKey="Veguilla, V" uniqKey="Veguilla V">V Veguilla</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stohr, K" uniqKey="Stohr K">K Stohr</name>
</author>
<author><name sortKey="Bucher, D" uniqKey="Bucher D">D Bucher</name>
</author>
<author><name sortKey="Colgate, T" uniqKey="Colgate T">T Colgate</name>
</author>
<author><name sortKey="Wood, J" uniqKey="Wood J">J Wood</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fulvini, Aa" uniqKey="Fulvini A">AA Fulvini</name>
</author>
<author><name sortKey="Ramanunninair, M" uniqKey="Ramanunninair M">M Ramanunninair</name>
</author>
<author><name sortKey="Le, J" uniqKey="Le J">J Le</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="O Eill, E" uniqKey="O Eill E">E O’Neill</name>
</author>
<author><name sortKey="Donis, Ro" uniqKey="Donis R">RO Donis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gubareva, Lv" uniqKey="Gubareva L">LV Gubareva</name>
</author>
<author><name sortKey="Wood, Jm" uniqKey="Wood J">JM Wood</name>
</author>
<author><name sortKey="Meyer, Wj" uniqKey="Meyer W">WJ Meyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gambaryan, As" uniqKey="Gambaryan A">AS Gambaryan</name>
</author>
<author><name sortKey="Matrosovich, Ty" uniqKey="Matrosovich T">TY Matrosovich</name>
</author>
<author><name sortKey="Philipp, J" uniqKey="Philipp J">J Philipp</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Suzuki, Y" uniqKey="Suzuki Y">Y Suzuki</name>
</author>
<author><name sortKey="Kato, H" uniqKey="Kato H">H Kato</name>
</author>
<author><name sortKey="Naeve, Cw" uniqKey="Naeve C">CW Naeve</name>
</author>
<author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Jadhao, Sj" uniqKey="Jadhao S">SJ Jadhao</name>
</author>
<author><name sortKey="Achenbach, J" uniqKey="Achenbach J">J Achenbach</name>
</author>
<author><name sortKey="Swayne, De" uniqKey="Swayne D">DE Swayne</name>
</author>
<author><name sortKey="Donis, R" uniqKey="Donis R">R Donis</name>
</author>
<author><name sortKey="Cox, N" uniqKey="Cox N">N Cox</name>
</author>
<author><name sortKey="Matsuoka, Y" uniqKey="Matsuoka Y">Y Matsuoka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ulmer, Jb" uniqKey="Ulmer J">JB Ulmer</name>
</author>
<author><name sortKey="Valley, U" uniqKey="Valley U">U Valley</name>
</author>
<author><name sortKey="Rappuoli, R" uniqKey="Rappuoli R">R Rappuoli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brown, Le" uniqKey="Brown L">LE Brown</name>
</author>
<author><name sortKey="Murray, Jm" uniqKey="Murray J">JM Murray</name>
</author>
<author><name sortKey="White, Do" uniqKey="White D">DO White</name>
</author>
<author><name sortKey="Jackson, Dc" uniqKey="Jackson D">DC Jackson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Takahashi, T" uniqKey="Takahashi T">T Takahashi</name>
</author>
<author><name sortKey="Hashimoto, A" uniqKey="Hashimoto A">A Hashimoto</name>
</author>
<author><name sortKey="Maruyama, M" uniqKey="Maruyama M">M Maruyama</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gambaryan, As" uniqKey="Gambaryan A">AS Gambaryan</name>
</author>
<author><name sortKey="Matrosovich, Mn" uniqKey="Matrosovich M">MN Matrosovich</name>
</author>
<author><name sortKey="Bender, Ca" uniqKey="Bender C">CA Bender</name>
</author>
<author><name sortKey="Kilbourne, Ed" uniqKey="Kilbourne E">ED Kilbourne</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guarnaccia, T" uniqKey="Guarnaccia T">T Guarnaccia</name>
</author>
<author><name sortKey="Carolan, La" uniqKey="Carolan L">LA Carolan</name>
</author>
<author><name sortKey="Maurer Stroh, S" uniqKey="Maurer Stroh S">S Maurer-Stroh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xu, R" uniqKey="Xu R">R Xu</name>
</author>
<author><name sortKey="De Vries, Rp" uniqKey="De Vries R">RP de Vries</name>
</author>
<author><name sortKey="Zhu, X" uniqKey="Zhu X">X Zhu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, H" uniqKey="Yang H">H Yang</name>
</author>
<author><name sortKey="Carney, Pj" uniqKey="Carney P">PJ Carney</name>
</author>
<author><name sortKey="Chang, Jc" uniqKey="Chang J">JC Chang</name>
</author>
<author><name sortKey="Villanueva, Jm" uniqKey="Villanueva J">JM Villanueva</name>
</author>
<author><name sortKey="Stevens, J" uniqKey="Stevens J">J Stevens</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hensley, Se" uniqKey="Hensley S">SE Hensley</name>
</author>
<author><name sortKey="Das, Sr" uniqKey="Das S">SR Das</name>
</author>
<author><name sortKey="Bailey, Al" uniqKey="Bailey A">AL Bailey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name>
</author>
<author><name sortKey="Baz, M" uniqKey="Baz M">M Baz</name>
</author>
<author><name sortKey="Lu, J" uniqKey="Lu J">J Lu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ramanunninair, M" uniqKey="Ramanunninair M">M Ramanunninair</name>
</author>
<author><name sortKey="Le, J" uniqKey="Le J">J Le</name>
</author>
<author><name sortKey="Onodera, S" uniqKey="Onodera S">S Onodera</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mitnaul, Lj" uniqKey="Mitnaul L">LJ Mitnaul</name>
</author>
<author><name sortKey="Matrosovich, Mn" uniqKey="Matrosovich M">MN Matrosovich</name>
</author>
<author><name sortKey="Castrucci, Mr" uniqKey="Castrucci M">MR Castrucci</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Myers, Jl" uniqKey="Myers J">JL Myers</name>
</author>
<author><name sortKey="Wetzel, Ks" uniqKey="Wetzel K">KS Wetzel</name>
</author>
<author><name sortKey="Linderman, Sl" uniqKey="Linderman S">SL Linderman</name>
</author>
<author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name>
</author>
<author><name sortKey="Sullivan, Cb" uniqKey="Sullivan C">CB Sullivan</name>
</author>
<author><name sortKey="Hensley, Se" uniqKey="Hensley S">SE Hensley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wagner, R" uniqKey="Wagner R">R Wagner</name>
</author>
<author><name sortKey="Wolff, T" uniqKey="Wolff T">T Wolff</name>
</author>
<author><name sortKey="Herwig, A" uniqKey="Herwig A">A Herwig</name>
</author>
<author><name sortKey="Pleschka, S" uniqKey="Pleschka S">S Pleschka</name>
</author>
<author><name sortKey="Klenk, Hd" uniqKey="Klenk H">HD Klenk</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Géorgie (États-Unis)</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Ridenour, Callie" sort="Ridenour, Callie" uniqKey="Ridenour C" first="Callie" last="Ridenour">Callie Ridenour</name>
</region>
<name sortKey="Balish, Amanda" sort="Balish, Amanda" uniqKey="Balish A" first="Amanda" last="Balish">Amanda Balish</name>
<name sortKey="Barr, John R" sort="Barr, John R" uniqKey="Barr J" first="John R" last="Barr">John R. Barr</name>
<name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name>
<name sortKey="Cox, Nancy J" sort="Cox, Nancy J" uniqKey="Cox N" first="Nancy J" last="Cox">Nancy J. Cox</name>
<name sortKey="Davis, Charles" sort="Davis, Charles" uniqKey="Davis C" first="Charles" last="Davis">Charles Davis</name>
<name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O" last="Donis">Ruben O. Donis</name>
<name sortKey="Hossain, Jaber" sort="Hossain, Jaber" uniqKey="Hossain J" first="Jaber" last="Hossain">Jaber Hossain</name>
<name sortKey="Johnson, Adam" sort="Johnson, Adam" uniqKey="Johnson A" first="Adam" last="Johnson">Adam Johnson</name>
<name sortKey="Kim, Taejoong" sort="Kim, Taejoong" uniqKey="Kim T" first="Taejoong" last="Kim">Taejoong Kim</name>
<name sortKey="Mateu Petit, Guaniri" sort="Mateu Petit, Guaniri" uniqKey="Mateu Petit G" first="Guaniri" last="Mateu-Petit">Guaniri Mateu-Petit</name>
<name sortKey="Santana, Wanda" sort="Santana, Wanda" uniqKey="Santana W" first="Wanda" last="Santana">Wanda Santana</name>
<name sortKey="Villanueva, Julie" sort="Villanueva, Julie" uniqKey="Villanueva J" first="Julie" last="Villanueva">Julie Villanueva</name>
<name sortKey="Williams, Tracie L" sort="Williams, Tracie L" uniqKey="Williams T" first="Tracie L" last="Williams">Tracie L. Williams</name>
<name sortKey="Winne, Emily" sort="Winne, Emily" uniqKey="Winne E" first="Emily" last="Winne">Emily Winne</name>
<name sortKey="Winne, Emily" sort="Winne, Emily" uniqKey="Winne E" first="Emily" last="Winne">Emily Winne</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000576 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000576 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:4548996 |texte= Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25962412" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
This area was generated with Dilib version V0.6.33. |